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Updated: Feb 28, 2026

Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training
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Evaluation of cardiac troponin I stability in blood sample using the AccuTnI+3 assay

M Nowoczyn1, C Lefevre, F Grandhomme

  • 1Department of Biochemistry, CHU de Caen, Caen, France. nowoczyn-m@chu-caen.fr.

European Review for Medical and Pharmacological Sciences
|June 16, 2017
PubMed

Insights

Cardiac troponin Ic (cTnI) levels remain stable for up to 4 hours after blood collection, supporting extended transport times for laboratory analysis. This finding is crucial for accurate cardiac injury detection outside emergency settings.

Area of Science:

  • Biochemistry
  • Clinical Diagnostics
  • Cardiology

Background:

  • Cardiac troponins are key biomarkers for detecting cardiac injury.
  • Current guidelines recommend <2h transport time for troponin analysis.
  • Non-emergency settings often exceed these transport recommendations.

Purpose of the Study:

  • To evaluate the in vitro stability of cardiac troponin Ic (cTnI).
  • To assess cTnI stability at 4 hours post-vein puncture compared to <2 hours.
  • To determine stability across a wide range of cTnI concentrations using the Beckman Coulter AccuTnI+3 assay.

Main Methods:

  • Analyzed 95 patient blood samples at two time points: <2 hours and 4 hours post-vein puncture.
  • Utilized the Beckman Coulter AccuTnI+3 assay for cTnI measurement.
  • Calculated correlation and evaluated bias using Bland-Altman analysis.

Main Results:

  • No significant differences in cTnI values were observed between the <2 hour and 4 hour analysis intervals.
  • Analytical reproducibility was maintained throughout the study.
  • Bland-Altman analysis showed minimal bias within acceptable limits.

Conclusions:

  • The time between vein puncture and cTnI analysis can be safely extended to 4 hours.
  • This extended timeframe supports troponin testing in non-emergency and remote settings.
  • Findings validate the reliability of cTnI measurements beyond the standard <2h recommendation.
Abstract

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