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Updated: Feb 28, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies
Pierre Gantner1, Adeline Mélard2, Florence Damond3
1Laboratoire de Virologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
Background:
Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter-laboratory reproducibility of total HIV-1-DNA quantification.
Methods:
Ten laboratories of the ANRS-AC11 working group participated by quantifying HIV-DNA with a real-time qPCR assay (Biocentric) in four samples (QCMD).
Results:
Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1
Conclusions:
Results of this QC validate the feasibility of multicenter studies using this standardized assay.
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