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A highly sensitive and widely adaptable plasmonic aptasensor using berberine for small-molecule detection.

Jin-Ho Park1, Ju-Young Byun2, Hyungjun Jang1

  • 1Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of Korea.

Biosensors & Bioelectronics
|June 16, 2017
PubMed
Summary

This study enhances localized surface plasmon resonance (LSPR) biosensors for sensitive small-molecule detection. Berberine (BER) significantly boosts the LSPR signal, enabling highly accurate quantification of various analytes.

Keywords:
AptamerBerberineEnhancementGold nanorod (GNR)Localized surface plasmon resonance (LSPR)Small molecule

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Area of Science:

  • Nanotechnology
  • Biosensing
  • Analytical Chemistry

Background:

  • Localized surface plasmon resonance (LSPR) biosensors offer label-free detection but suffer from low sensitivity and limited dynamic range.
  • G-quadruplex (GQx) structures can be targeted by specific binders, presenting an opportunity to amplify biosensor signals.

Purpose of the Study:

  • To develop a highly sensitive plasmonic aptasensor for small-molecule detection by leveraging G-quadruplex (GQx) structure-binding interactions.
  • To investigate the signal enhancement capabilities of known GQx binders on a plasmonic aptasensor.

Main Methods:

  • A plasmonic aptasensor was designed using gold nanorods.
  • Six G-quadruplex (GQx) binders were screened for their ability to enhance the LSPR signal upon aptamer/target binding.
  • Berberine (BER) was identified as the optimal enhancer, and its interaction with the GQx structure was confirmed.

Main Results:

  • Berberine (BER) significantly enhanced the LSPR peak shift, leading to a 1000-fold increase in sensitivity compared to BER-free conditions.
  • The developed aptasensor achieved limits of detection (LOD) in the picomolar range for ochratoxin A, aflatoxin B1, adenosine triphosphate, and potassium ions.
  • A wide dynamic range (10 pM to 10 μM) and effective quantitative analysis in real samples were demonstrated.

Conclusions:

  • The integration of G-quadruplex (GQx) binders, specifically berberine (BER), dramatically improves the sensitivity and dynamic range of LSPR aptasensors.
  • This enhanced plasmonic aptasensor provides a robust platform for highly sensitive and quantitative detection of various small molecules.