Molecular characterization of Blastocystis from pigs in Shaanxi province of China

Blastocystis is an enteric eukaryote of mystery for its ubiquitous presence in animals and humans worldwide and a broad diversity genetically. The animals have been suggested to be an important reservoir to transmit Blastocystis to humans because of high colonization frequency and the presence of zoonotic subtypes. In the present study, the prevalence and subtypes of Blastocystis in pigs in Shaanxi province of China were determined using the molecular technique based on the small subunit rRNA (SSU rRNA) gene fragment. Of 560 pig faecal samples collected from different geographical origins, 419 (74.8%) were positive for Blastocystis colonization. The prevalence was significant affected by the age and the geographical origin. Four subtypes, including three zoonotic (ST1, ST3 and ST5) and one animal specific (ST10) subtypes, were identified. To our knowledge, this study provides the first run-through information for colonization of Blastocystis in pigs in China.