Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

10.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.3K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

37.8K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.8K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.7K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.7K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.4K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fabry disease cardiomyopathy: Time for a closer heart rhythm monitoring?

Heart failure reviews·2026
Same author

A Chimeric Airway Model Enables Evaluation of Essential Genes In Vivo.

American journal of respiratory cell and molecular biology·2026
Same author

Impact of migalastat therapy on corneal deposits in a female with Fabry disease: A case report.

Molecular genetics and metabolism reports·2026
Same author

CFTR facilitates fluid secretion by ferret alveolar type 2 cells.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2026
Same author

Activation of the impaired NAMPT/SIRT7/SOD2 axis restores alveolar progenitor cell renewal in idiopathic pulmonary fibrosis.

The Journal of clinical investigation·2026
Same author

Syndecan-1 Promotes Alveolar Type 2 Epithelial Cell Senescence during Lung Fibrosis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Feb 28, 2026

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
09:45

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

9.4K

Sin3a regulates epithelial progenitor cell fate during lung development.

Changfu Yao1, Gianni Carraro1, Bindu Konda1

  • 1Lung and Regenerative Medicine Institutes, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Development (Cambridge, England)
|June 17, 2017
PubMed
Summary

The co-repressor Sin3a is essential for lung development. Its absence causes progenitor cells to senesce, leading to respiratory failure in newborn mice.

Keywords:
Epithelial-mesenchymal signalingForegut endodermG1 arrestMouseProgenitor cell fateSin3ap21/Cdkn1a

More Related Videos

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
12:31

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Published on: May 5, 2016

9.1K
Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.8K

Related Experiment Videos

Last Updated: Feb 28, 2026

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
09:45

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

9.4K
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
12:31

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Published on: May 5, 2016

9.1K
Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.8K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Mechanisms regulating tissue-specific progenitor maintenance and differentiation during development are not well understood.
  • The role of co-repressor proteins in embryonic development is an area of active research.

Purpose of the Study:

  • To investigate the role of the co-repressor protein Sin3a in early lung endoderm development.
  • To elucidate the cellular and molecular consequences of Sin3a loss in developing mouse lungs.

Main Methods:

  • Utilized mouse models with targeted loss of Sin3a in the early foregut endoderm.
  • Analyzed lung development, branching morphogenesis, and epithelial cell states.
  • Investigated cell cycle regulation and epithelial-mesenchymal signaling pathways.

Main Results:

  • Loss of Sin3a resulted in severe lung development defects, including failed branching morphogenesis and proximal lung endoderm atrophy.
  • Embryonic lung epithelial progenitor cells entered a senescence-like state with G1 phase cell cycle arrest, partly due to CDKN1A and CDKN2C upregulation.
  • Sin3a deficiency disrupted mesodermal differentiation, indicating impaired epithelial-mesenchymal signaling.

Conclusions:

  • Sin3a is a critical regulator for early lung endoderm specification and differentiation.
  • Sin3a's function is essential for preventing progenitor cell senescence and maintaining lung epithelial integrity.
  • Aberrant epithelial-mesenchymal signaling is a consequence of Sin3a loss in lung development.