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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Published on: June 7, 2016
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The (pro)renin receptor and its interaction partners.
1Institute of Physiology, University Medicine of Greifswald, Greifswalder Str. 11 C, 17495, Karlsburg, Germany. joerg.peters@uni-greifswald.de.
Pflugers Archiv : European Journal of Physiology
|June 17, 2017
Summary
The prorenin receptor (PRR) is vital for cellular functions beyond its initial discovery. It acts as a crucial component of vesicular ATPases and Wnt signaling pathways, essential for development and cell biology.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The prorenin receptor (PRR) was initially identified for its role in binding renin and prorenin, potentially mediating pro-fibrotic effects.
- Therapeutic strategies targeting PRR aimed to inhibit these pro-fibrotic cascades, particularly in kidney disease.
- However, experimental evidence contradicted this concept, showing PRR overexpression was benign while its depletion was detrimental.
Purpose of the Study:
- To re-evaluate the function of the prorenin receptor (PRR) beyond its initially proposed role.
- To investigate the actual biological significance of PRR in cellular processes.
- To understand the diverse roles of PRR in cellular machinery and signaling.
Main Methods:
- Investigated the cellular localization and interactions of the prorenin receptor (PRR).
- Analyzed the effects of PRR manipulation on cellular processes and organismal viability.
- Examined the role of PRR in the context of vesicular ATPases and Wnt signaling pathways.
Main Results:
- The prorenin receptor (PRR) is not primarily a mediator of pro-fibrotic signals but has essential physiological roles.
- PRR functions as a critical accessory subunit of vesicular ATPases (v-ATPases), essential for their assembly.
- PRR is integral to both canonical and non-canonical Planar Cell Polarity (PCP) Wnt signaling pathways.
Conclusions:
- The prorenin receptor (PRR), also known as ATP6AP2, possesses critical functions unrelated to its initial discovery.
- PRR is indispensable for lysosomal functions, including endocytosis, secretion, and autophagy.
- PRR plays vital roles in cell division, differentiation, embryonic development, organogenesis, and stem cell biology.
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