miR-2909 regulates ISGylation system via STAT1 signalling through negative regulation of SOCS3 in prostate cancer

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Updated: Feb 28, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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Shiekh Gazalla Ayub1, Deepak Kaul1

  • 1Department of Experimental Medicine and Biotechnology, Post-Graduate Institute of Medical Sciences and Research, Chandigarh, India.

Andrology
|June 17, 2017
PubMed

Insights

MicroRNA-2909 (miR-2909) promotes prostate cancer by upregulating immune-related genes and STAT1. Inhibiting this pathway may offer new prostate cancer (PCa) treatment strategies.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Tumor cells evade immune surveillance for progression.
  • Understanding cancer-immunity interactions can yield novel cancer treatments, including for prostate cancer (PCa).

Purpose of the Study:

  • To investigate the role of miR-2909 in prostate cancer progression and its interaction with immune and signaling pathways.
  • To elucidate the regulatory mechanisms of miR-2909 in PCa.

Main Methods:

  • Analysis of miR-2909's effect on interferon-stimulating genes (ISGs) and the ISGylation system via STAT1.
  • Investigating miR-2909's regulation of SOCS3 and Type 1 interferon (IFN) production.
  • Examining the impact of ISGylation inhibition on PCa cell proliferation.
  • Assessing the influence of androgens and TGF-β signaling on ISGylation and miR-2909 activity.

Main Results:

  • miR-2909 upregulates ISGs and the ISGylation system through STAT1 by negatively regulating SOCS3, independent of Type 1 IFN production.
  • Inhibition of ISGylation significantly reduced PCa cell proliferation.
  • Androgens negatively regulate ISGylation in LNCaP cells via androgen receptor signaling, independent of miR-2909.
  • miR-2909 suppresses TGF-β mediated SMAD3 signaling by inducing SMAD7 through enhanced STAT1 expression.

Conclusions:

  • miR-2909 plays a significant role in prostate carcinogenesis.
  • Modulation of the ISGylation system and TGF-β signaling via STAT1 by miR-2909 are key mechanisms in PCa.
  • Targeting miR-2909 or its downstream pathways presents a potential therapeutic strategy for prostate cancer.
Keywords:
ISG15SOCS3prostate cancer

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