Related Experiment Video
Updated: Feb 28, 2026
13:38
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Published on: January 27, 2012
14.7K
SnapShot: Phosphoregulation of Mitosis
Andrew Burgess1, Jenny Vuong2, Samuel Rogers3
1Garvan Institute of Medical Research, Sydney NSW 2010, Australia; St. Vincent's Clinical School, UNSW, Sydney NSW 2010, Australia.
Cell
|June 17, 2017
Summary
Accurate cell division (mitosis) prevents diseases like cancer. This study reveals key phosphorylation events and their timing, crucial for controlling cell division, using mass spectrometry. This advances understanding of cell cycle regulation.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Mitosis ensures accurate genome duplication and cell division.
- Dysregulation of mitosis can lead to proliferative diseases, including cancer.
- A complex network of kinases and phosphatases controls thousands of phosphorylation events during mitosis.
Purpose of the Study:
- To identify and characterize key phosphorylation events regulating mitosis.
- To understand the temporal dynamics of these regulatory events.
- To advance the understanding of cell cycle control mechanisms.
Main Methods:
- Utilized mass spectrometry to analyze phosphorylation events.
- Investigated the identity, magnitude, and temporal regulation of phosphoevents.
- Focused on key regulators of the mitotic process.
Main Results:
- Identified specific phosphoevents critical for mitotic regulation.
- Characterized the precise timing and magnitude of these events.
- Provided new insights into the kinase-phosphatase network's role in mitosis.
Conclusions:
- Phosphorylation events are precisely timed regulators of mitosis.
- Understanding these events is crucial for preventing errors in cell division.
- This work contributes to the fundamental knowledge of cell cycle control and disease prevention.
Related Concept Videos
01:45Positive Regulator Molecules
136.8K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.8K
02:39Positive Regulator Molecules
7.0K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
7.0K
01:02Phosphorylation
54.9K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
54.9K
01:28The Cell Cycle Control System
6.1K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
6.1K
02:11The Cell Cycle Control System
14.7K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
14.7K
01:27Molecular Factors Affecting Cell Division
4.0K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
4.0K