Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

931
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
931

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting in vivo performance of piroxicam and apalutamide drug nanocrystals from in vitro flux measurements combined with in silico modelling.

International journal of pharmaceutics·2026
Same author

Effect of HIFU frequency on gold removal efficiency from e-waste.

Scientific reports·2026
Same author

High-power burst and chirp amplifier for MHz ultrasonics.

The Review of scientific instruments·2025
Same author

Use of Prussian Blue pseudocapacitive properties to amplify the pulsed amperometric readout of biosensors.

Biosensors & bioelectronics·2025
Same author

Reasons for cancellation of total knee arthroplasty surgery in NHS hospitals in the UK.

The bone & joint journal·2025
Same author

Label free super resolution imaging with photonic nanojets from tunable tapered optical fibers.

Nanophotonics (Berlin, Germany)·2025
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

19.0K

3D Super-Resolution Optical Profiling Using Microsphere Enhanced Mirau Interferometry.

Ivan Kassamakov1, Sylvain Lecler2, Anton Nolvi3

  • 1University of Helsinki, Helsinki, Finland. ivan.kassamakov@helsinki.fi.

Scientific Reports
|June 18, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a microsphere-enhanced interferometric microscope for high-resolution 3D imaging of Blu-ray disc grooves. This novel technique achieves nanometer-scale axial resolution and ~112nm lateral resolution, with potential applications in biomedicine and nanotechnology.

More Related Videos

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.5K
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

10.2K

Related Experiment Videos

Last Updated: Feb 28, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

19.0K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.5K
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

10.2K

Area of Science:

  • Optical microscopy
  • Nanotechnology
  • Materials science

Background:

  • High-resolution imaging is crucial for analyzing micro- and nanoscale structures.
  • Interferometric microscopy offers non-contact surface profiling capabilities.
  • Microspheres can generate photonic nanojets, potentially enhancing optical resolution.

Purpose of the Study:

  • To develop and validate a novel 3D imaging technique for Blu-ray disc grooves.
  • To investigate the role of photonic nanojets in enhancing microscopic resolution.
  • To achieve nanometer-scale resolution for surface characterization.

Main Methods:

  • Utilized a Mirau interferometric microscope equipped with a microsphere as a photonic nanojet source.
  • Performed quantitative 3D imaging of writable Blu-ray Disc grooves.
  • Developed computational models to simulate photonic nanojet interaction with the disc surface.

Main Results:

  • Achieved axial resolution of a few nanometers and lateral resolution of approximately 112 nm.
  • Demonstrated agreement between simulation results and experimental data.
  • Identified the potential role of coherence in achieving lateral super-resolution.

Conclusions:

  • The microsphere-assisted interferometric system provides high-resolution 3D imaging of microstructures.
  • Photonic nanojets contribute to understanding the imaging process and achieving enhanced resolution.
  • This imaging modality holds promise for applications in biomedicine and nanotechnology.