Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Waste heat promoted ammonia recovery from sludge lysate using a MES-TMCS system: thermal-electric-microbial synergies.

Water research·2026
Same author

1,3-Dichloro-5,5-dimethylhydantoin (DCDMH)-Driven Sludge Pretreatment for Organic Carbon Valorization: Mechanistic Insights into Controlled Oxidative Disruption and Hormesis-Mediated Metabolic Reshaping.

Environmental science & technology·2026
Same author

Metagenomic-metabolomic integration elucidates stage-specific dynamics of microbial communities and metabolites driving pork spoilage in commercial supply chains.

Food research international (Ottawa, Ont.)·2026
Same author

Enhancing waste activated sludge dewatering and simultaneously inhibiting microbial viability by Fe(Ⅱ)-activated 1,3-Dichloro-5,5-dimethylhydantoin (Fe(Ⅱ)/DCDMH) process.

Water research·2026
Same author

Shaping of microbial community structure and function by effluent-derived dissolved organic matter in a receiving river across seasons.

Eco-Environment & Health·2026
Same author

Varied mixed carbon source ratios affect nitrous oxide emissions in aerobic granular sludge systems.

Bioresource technology·2026

Related Experiment Video

Updated: Feb 28, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K

Colorimetric sensor array for protein discrimination based on different DNA chain length-dependent gold nanoparticles

Xiangcong Wei1, Yuxian Wang1, Yingxin Zhao1

  • 1Department of Chemistry, Capital Normal University, Beijing 100048, China.

Biosensors & Bioelectronics
|June 18, 2017
PubMed
Summary

This study presents a simple colorimetric sensor array using DNA to detect and differentiate proteins. The sensor array achieved 100% accuracy in distinguishing proteins and analyzing human serum samples.

Keywords:
ColorimetricDiscriminationGold nanoparticlesProteinsSensor array

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K

Related Experiment Videos

Last Updated: Feb 28, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.2K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.8K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Protein detection is crucial in diagnostics and research.
  • Existing methods for protein analysis can be complex and costly.
  • Development of rapid, sensitive, and cost-effective protein detection platforms is needed.

Purpose of the Study:

  • To develop a facile colorimetric sensor array for protein detection and discrimination.
  • To utilize DNA as nonspecific receptors for triggering differential aggregation of gold nanoparticles (AuNPs).
  • To demonstrate the array's capability in distinguishing multiple proteins and analyzing human serum samples.

Main Methods:

  • Fabrication of a sensor array using two single-stranded DNA (ssDNA) sequences as receptors.
  • Utilizing DNA-protected gold nanoparticles (AuNPs) that exhibit varied aggregation behaviors and color changes in response to different proteins under high salt concentrations.
  • Employing linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA) for data analysis of combinatorial colorimetric responses.

Main Results:

  • Successful discrimination of 12 different proteins at the 50 nM level using the naked eye.
  • Demonstrated ability to discriminate proteins present in human serum.
  • Achieved 100% accuracy in discriminating complex protein mixtures and unknown samples.

Conclusions:

  • The developed DNA-based colorimetric sensor array offers a simple and effective method for protein detection and discrimination.
  • The sensor array shows high sensitivity and accuracy, with potential applications in clinical diagnostics and biological research.
  • This approach provides a promising platform for label-free, visual detection of complex biological samples.