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Oxygen and tissue culture affect placental gene expression
1University of West London, Paragon House, Boston Manor Road, Brentford, Middlesex, TW8 9GA, UK.
Placenta
|June 19, 2017
Summary
Placental explant culture significantly alters gene expression, inducing stress and apoptosis. Atmospheric oxygen exacerbates these changes, impacting key biological pathways and diminishing trophoblast function.
Area of Science:
- Reproductive Biology
- Genomics
- Cellular Biology
Background:
- Placental explant culture is crucial for studying placental development and function.
- Understanding gene expression changes in cultured explants is vital.
Purpose of the Study:
- To investigate placental gene expression differences under tissue culture conditions.
- To compare gene expression at atmospheric versus physiologic oxygen concentrations.
Main Methods:
- Collected term placental explants from uncomplicated pregnancies.
- Analyzed placental transcriptomic expression using Affymetrix GeneChip arrays.
Main Results:
- Identified gene subsets regulating stress response, apoptosis, cell growth, and viability.
- Tissue culture induced significant gene expression changes, irrespective of oxygen levels.
- Atmospheric oxygen culture amplified stress responses, inflammatory markers, and apoptosis, while reducing trophoblast proliferation and viability.
Conclusions:
- Gene expression patterns in placental explants change significantly upon culture.
- Culturing explants at atmospheric oxygen promotes a stressed, pro-inflammatory, and apoptotic transcriptomic profile.
Keywords:
Atmospheric oxygen concentrationGene expressionPhysiologic oxygen concentrationPlacentaTissue cultureMore Related Videos
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