Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Therapeutic potential of curcumin-integrated starch biomaterials in wound regeneration.

Journal of materials science. Materials in medicine·2026

Same author

Maleic acid assisted facile plasmonic chip for assessment of adulterants in cattle milk.

RSC advances·2026

Same author

Machine learning enhanced optical spectroscopy for breast cancer diagnosis: A review.

Lasers in medical science·2026

Same author

Advancing collagen-related pathology assessment through second-harmonic generation imaging.

Journal of microscopy·2026

Same author

Deep Learning Integration in Optical Microscopy: Advancements and Applications.

Microscopy research and technique·2026

Same author

Deciphering the Worst Pattern of Invasion in Oral Cancer: Integrative Clinical, Pathological, and Multimodal Nonlinear Optical Imaging Insights.

Laboratory investigation; a journal of technical methods and pathology·2025

Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026

Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026

Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026

Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026

Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026

Same journal

An automated workflow for quantifying the formation of synuclein aggregates in human dopaminergic neurons.

Methods (San Diego, Calif.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Polarization resolved second harmonic microscopy.

Nirmal Mazumder¹, Gitanjal Deka², Wei-Wen Wu³

¹Department of Biophysics, School of Life Sciences, Manipal University, Manipal 576104, India.

Methods (San Diego, Calif.)

|June 19, 2017

Summary

This summary is machine-generated.

Second harmonic (SH) microscopy uses polarized light to reveal molecular structure in tissues without labels. This review details polarization-resolved SH microscopy techniques for advanced biological imaging and diagnostics.

Keywords:

Chirality Fluorescence lifetime imaging (FLIM)Optical scanning microscopy Polarization Second harmonic Stokes vector

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Related Experiment Videos

Last Updated: Feb 28, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Area of Science:

Biophotonics
Molecular Imaging
Microscopy

Background:

Second harmonic (SH) microscopy is a label-free imaging technique utilizing endogenous contrast.
Its multiphoton nature enables optical sectioning, deep tissue penetration, and non-invasive observation.
SH microscopy can probe molecular structure and orientation based on light polarization and tissue properties.

Purpose of the Study:

To review polarization-resolved techniques in SH microscopy.
To highlight advancements in imaging molecular structure, orientation, and chirality.
To discuss applications in biological tissue analysis and in vivo diagnostics.

Main Methods:

Focus on Stokes vector-based polarimetry for SH microscopy.
Elucidation of polarization-resolving techniques and instrumentation.
Overview of SH anisotropy measurements and SH circular dichroism.

Main Results:

Demonstration of Stokes vector resolving for imaging molecular structure and orientation.
Characterization of 3-D structural chirality using SH circular dichroism.
Integration with fluorescence lifetime imaging (FLIM) for wound healing diagnosis.

Conclusions:

Polarization-resolved SH microscopy offers unique insights into molecular and structural properties of biological tissues.
Advancements enable detailed analysis of molecular orientation, symmetry, and chirality.
Future research directions include exploring complex tissues and refining diagnostic applications.