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Updated: Feb 28, 2026

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC

Siliang Xue1, Lang He2, Xiao Zhang3

  • 1Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Archives of Medical Research
|June 20, 2017
PubMed
Abstract

Insights

Jagged1/Notch3 signaling is expressed in triple-negative breast cancer (TNBC). Jagged1 predicts poor prognosis, and Jagged1/Notch3 may be a therapeutic target for TNBC angiogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • The Jagged1/Notch3 signaling pathway is crucial for breast cancer angiogenesis.
  • Its role in triple-negative breast cancer (TNBC) remains largely uncharacterized.
  • This study investigates Jagged1/Notch3 expression and its clinical significance in TNBC.

Purpose of the Study:

  • To determine Jagged1/Notch3 mRNA and protein expression in TNBC.
  • To analyze correlations between Jagged1/Notch3 and clinicopathological features, prognosis, and VEGF.
  • To evaluate Jagged1/Notch3 as a potential therapeutic target in TNBC.

Main Methods:

  • Quantitative real-time PCR (Q-RT-PCR) and Western blotting for mRNA and protein levels.
  • Immunohistochemistry for Jagged1/Notch3, VEGF, and CD34 (microvessel density marker).
  • Statistical analysis to assess correlations and prognostic significance.

Main Results:

  • Jagged1/Notch3 mRNA and protein expression were concordant.
  • Positive Jagged1 expression correlated with poorer disease-free survival (DFS) and overall survival (OS) in TNBC patients.
  • Jagged1 was an independent predictor of OS.
  • VEGF expression correlated positively with microvessel density (MVD).
  • MVD was significantly associated with both Jagged1 and Notch3 expression.
  • Jagged1/Notch3 influenced DFS and OS in TNBC patients with positive VEGF expression.

Conclusions:

  • Jagged1/Notch3 is expressed in TNBC at both mRNA and protein levels.
  • Jagged1 serves as an independent predictor of poor prognosis in TNBC.
  • A potential crosstalk exists between Jagged1/Notch3 and VEGF in TNBC angiogenesis.
  • Jagged1/Notch3 represents a promising signaling pathway and therapeutic target for TNBC progression and neovascularization.
Keywords:
AngiogenesisJagged1Notch3Triple-negative breast cancer

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