[Effect of trehalose on survival rate for fat cells after cryopreservation]
Ying Deng1, Shaoqian Liu1, Hongju Xie1
1Department of Medical Cosmetology, First Affiliated Hospital of University of South China, Hengyang Hunan 421001, China.
Objective:
To explore effects of trehalose as a cryopreserve agent on survival rate of fatty tissue after cryopreservation. Methods: The liposuction was used on the abdomen of adult female. After centrifugation and purification, adipose was randomized into the following three groups, the trehalose group, the fetal bovine serum (FBS)+10%DMSO group and the physiological saline group. The specimens were cryopreserved at -196 ℃ for 3 months and then the HE staining, glucose transfer method and CK method were used to detect the cell survival rate in each group. Results: The activity of adipose in the trehalose group and FBS+10%DMSO group adipose was higher than that in the physiological saline group (P<0.05); while there was no significant difference between the trehalose group and FBS+10%DMSO group (P>0.05). Conclusion: As cryoprotectant, trehalose could keep fat cell viability, and adipose tissue can be used for clinical transplantation after 3 months' freezing.
