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Steam quality and effective sterilization.

R S Sedlacek, E F Rose

    Progress in Clinical and Biological Research
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Wet steam from a distant utility caused ineffective sterilization of mouse feed pellets, leading to Clostridium perfringens type D contamination and diarrhea in mice. Consistent sterility requires dry steam.

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    Area of Science:

    • Microbiology
    • Veterinary Science
    • Sterilization Technology

    Background:

    • Sterilization of animal feed is critical for preventing disease transmission in research settings.
    • Commercial steam utilities can experience issues with steam quality (e.g., wet steam) due to distance and system usage.
    • Ineffective sterilization poses a significant risk to animal health and experimental integrity.

    Purpose of the Study:

    • To investigate the efficacy of steam sterilization for mouse feed pellets using a distant commercial steam utility.
    • To identify the causes of sterilization failures.
    • To assess the microbiological impact of contaminated feed on laboratory mice.

    Main Methods:

    • Direct physical measurements using a portable steam calorimeter to assess steam quality.

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  • Direct microbiological testing of autoclaved feed pellets.
  • Indirect microbiological testing of feces from mice consuming the feed.

    • Main Results:

    • Colorimetric measurements confirmed steam contained >5% entrained water (wet steam).
    • Consistent sterility of feed pellets could not be achieved with wet steam, even at 126°C for 60 minutes.
    • Clostridium perfringens type D was the predominant isolate from contaminated pellets.
    • Mice fed wet steam-treated pellets developed diarrhea and shed C. perfringens type D in their feces.

    Conclusions:

    • Wet steam from distant commercial utilities is insufficient for reliable sterilization of mouse feed pellets.
    • Contaminated feed poses a health risk to laboratory animals, leading to gastrointestinal distress and pathogen shedding.
    • Ensuring dry steam quality is essential for effective sterilization and maintaining animal model integrity.