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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
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Microcontact-BSA imprinted capacitive biosensor for real-time, sensitive and selective detection of BSA
Gizem Ertürk1, Dmitriy Berillo1, Martin Hedström1
1Department of Biotechnology, Lund University, Lund, Sweden.
Biotechnology Reports (Amsterdam, Netherlands)
|June 20, 2017
Summary
A new method uses microcontact imprinting and capacitive biosensors to detect Bovine Serum Albumin (BSA). This technique offers highly sensitive BSA detection with excellent stability and low cross-reactivity, paving the way for advanced biosensing applications.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Bovine Serum Albumin (BSA) is a crucial biomarker in various biological and medical applications.
- Sensitive and selective detection methods for BSA are essential for accurate diagnostics and research.
- Existing biosensing techniques may face limitations in sensitivity, stability, or specificity.
Purpose of the Study:
- To develop a novel analytical method for highly sensitive Bovine Serum Albumin (BSA) detection.
- To combine microcontact imprinting with capacitive biosensor technology for enhanced performance.
- To evaluate the stability, selectivity, and limit of detection of the developed biosensor.
Main Methods:
- Preparation of protein stamps using glass cover slips for Bovine Serum Albumin (BSA) imprinting.
- Fabrication of microcontact-BSA imprinted gold electrodes via UV-polymerization with methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA).
- Real-time detection of BSA using the imprinted electrodes and a capacitive biosensor setup, with non-imprinted electrodes as reference.
Main Results:
- Achieved highly sensitive real-time BSA detection in the concentration range of 1.0 × 10-20 to 1.0 × 10-8 M.
- Established a limit of detection (LOD) as low as 1.0 × 10-19 M for BSA.
- Demonstrated low cross-reactivity towards Human Serum Albumin (HSA) (5%) and Immunoglobulin G (IgG) (3%), indicating high specificity.
- The imprinted electrodes maintained binding properties for over 70 assays during a 2-month period, showing excellent reusability and stability.
Conclusions:
- The integration of microcontact imprinting and capacitive biosensor technology provides a powerful platform for sensitive and selective BSA detection.
- The developed method exhibits superior sensitivity and stability compared to conventional techniques.
- This approach holds significant promise for future advancements in biosensing and diagnostic applications.
