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MicroRNA-508-5p suppresses metastasis in human gastric cancer by targeting S-phase kinase‑associated protein 2

Xiangguo Duan1, Jing Bai2, Jun Wei1

  • 1Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.

Molecular Medicine Reports
|June 20, 2017
PubMed

Insights

MicroRNA-508-5p suppresses gastric cancer (GC) progression by targeting the S-phase kinase-associated protein 2 (SKP2) oncogene. This microRNA inhibits cell proliferation, migration, and invasion, offering a potential therapeutic target for GC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • S-phase kinase-associated protein 2 (SKP2) is a significant oncogene frequently upregulated in gastric cancer (GC).
  • SKP2 upregulation in GC tissues shows an inverse correlation with microRNA (miR)‑508‑5p transcript levels.
  • Downregulation of miR‑508‑5p is associated with GC progression.

Purpose of the Study:

  • To investigate the functional impact of miR‑508‑5p on SKP2 expression and its role in gastric cancer cell metastasis.
  • To elucidate the mechanism by which miR‑508‑5p affects SKP2 and cellular processes in SGC‑7901 GC cells.

Main Methods:

  • Overexpression of miR‑508‑5p in SGC‑7901 GC cells.
  • Assays conducted: luciferase reporter assays, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and immunoblotting.
  • Evaluation of cell proliferation, migration, invasion, apoptosis, and cell cycle distribution.

Main Results:

  • Overexpression of miR‑508‑5p significantly inhibited proliferation, migration, and invasion of SGC‑7901 cells.
  • miR‑508‑5p induced apoptosis and cell cycle arrest at the G0/G1 phase.
  • miR‑508‑5p directly targets the SKP2 gene, downregulating its transcriptional and post-transcriptional expression.

Conclusions:

  • miR‑508‑5p plays a crucial role in suppressing gastric cancer progression and metastasis by targeting the SKP2 oncogene.
  • The findings suggest miR‑508‑5p as a potential therapeutic agent for gastric cancer.
  • miR‑508‑5p regulates SKP2 expression and impacts cell cycle control in gastric cancer.

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