Two simple media for biochemical experimentation with cultured procyclic Trypanosoma brucei

Two simple media are presented which are particularly well suited for biochemical experimentation with procyclic culture forms of Trypanosoma brucei. ME-83 is a simple semi-defined medium which supports active cell growth, and from which individual components can conveniently be deleted or replaced. HHP-84 is a fully defined minimal medium, which allows vigorous cell motility over extended times, but in which cell proliferation is not occurring.