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Updated: Feb 28, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Adaptive optics improves multiphoton super-resolution imaging.

Wei Zheng^1,2, Yicong Wu¹, Peter Winter¹

¹Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.

|June 20, 2017

Summary

This summary is machine-generated.

We enhanced multiphoton microscopy resolution and intensity using a nonlinear guide star and deformable mirror. This technique improves deep tissue imaging in various biological samples.

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Last Updated: Feb 28, 2026

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Published on: April 9, 2014

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Biomedical Optics
Microscopy
Biophysics

Background:

Multiphoton microscopy offers deep tissue imaging but suffers from optical aberrations.
Structured illumination microscopy enhances resolution but is sensitive to aberrations.

Purpose of the Study:

To improve resolution and signal intensity in multiphoton structured illumination microscopy.
To correct optical aberrations in deep biological tissues.

Main Methods:

Utilized a nonlinear guide star to measure optical aberrations.
Employed a deformable mirror for real-time aberration correction.
Applied the technique to various biological samples including cells, larvae, and Drosophila brains.

Main Results:

Achieved up to 40-fold increase in peak intensity.
Recovered resolution to 176 ± 10 nm laterally and 729 ± 39 nm axially.
Demonstrated effective aberration correction at depths of ~250 micrometers.

Conclusions:

The developed method significantly enhances the performance of multiphoton structured illumination microscopy.
This technique enables clearer and deeper imaging of biological structures.
Offers a valuable tool for advanced biological research and diagnostics.