Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 28, 2026

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
09:17

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

Published on: February 2, 2019

8.3K

Paper-based Invasion Assays for Quantifying Cellular Movement in Three-dimensional Tissue-like Structures.

C Chad Lloyd1, Matthew W Boyce1, Matthew R Lockett1,2

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

Current Protocols in Chemical Biology
|June 20, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selfish bacteria take up polysaccharides under deep ocean pressure: insights from in situ and ex situ measurements.

ISME communications·2026
Same author

Opportunities and challenges for analytical chemists: organ-on-chip devices as new approach methods (NAMs) for identifying potential toxins.

The Analyst·2026
Same author

Syngas Production at Si Hybrid Photoelectrodes Modified with Re(I) and Mn(I) Tricarbonyl Phenanthroline Complexes Containing Reactive Aryl Azide Groups.

ACS applied materials & interfaces·2026
Same author

Potential-Controlled Deposition of Multilayer CO<sub>2</sub> Reduction Catalyst Films onto Silicon Photoelectrodes Demonstrates Thickness-Dependent Catalytic Rates.

ACS applied materials & interfaces·2025
Same author

Immobilizing a Lehn-Type Catalyst with Nitrocyclocondensation Chemistries: CO<sub>2</sub> Reduction on Silicon Hybrid Photoelectrodes.

ACS applied materials & interfaces·2025
Same author

An analytical screening platform to differentiate acute and prolonged exposures of per- and polyfluoroalkyl substances on invasive cellular phenotypes.

Toxicological sciences : an official journal of the Society of Toxicology·2025

Researchers developed a 3D paper-based assay to study cancer cell invasion in tumor-like environments. This method allows for visualization and quantification of invasion, aiding in understanding cancer cell migration and the development of new therapies.

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Cell Biology

Background:

  • Understanding the chemical and environmental factors driving cancer cell invasion is crucial for developing effective cancer therapies.
  • Existing methods often fail to accurately replicate the complex tumor microenvironment, limiting the study of cancer cell invasion.

Purpose of the Study:

  • To develop and validate a novel three-dimensional (3D) paper-based invasion assay.
  • To simulate a tumor-like environment, specifically poorly vascularized tissue, for studying cancer cell invasion.
  • To enable experimental manipulation and probing of the chemical landscape influencing cancer cell behavior.

Main Methods:

  • Development of a 3D paper-based culture system.
  • Simulation of poorly vascularized tissue conditions within the assay.
Keywords:
chemotaxishypoxiainvasionoxygen gradientstumor microenvironment

More Related Videos

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
06:14

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Published on: March 12, 2018

6.5K
Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials
08:53

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

1.3K

Related Experiment Videos

Last Updated: Feb 28, 2026

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
09:17

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

Published on: February 2, 2019

8.3K
Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
06:14

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Published on: March 12, 2018

6.5K
Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials
08:53

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

1.3K
  • Utilizing different materials to control chemical gradients across the culture.
  • Visualization and quantification of cancer cell invasion through the engineered microenvironment.

    • Main Results:

    • The 3D paper-based invasion assay successfully simulates key aspects of the tumor microenvironment.
    • The assay allows for clear visualization and accurate quantification of cancer cell invasion.
    • The system is easy to assemble, enabling parallel performance of multiple invasion assays.

    Conclusions:

    • The developed 3D paper-based invasion assay provides a robust and accessible platform for studying cancer cell invasion.
    • This assay facilitates the evaluation of small molecule chemotactic potential in a more representative tissue microenvironment.
    • This technology can advance our understanding of the conditions promoting cancer cell metastasis and inform therapeutic strategies.