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Updated: Feb 28, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
Published on: April 2, 2015
A composite polynomial approach for analyzing the indefinite self-association of macromolecules studied by
1Institute for Protein Research, Osaka University, 3-1- Yamada-oka, Suita, Osaka 565-0871, Japan; Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia..
Abstract:
A different approach is described for analyzing sedimentation equilibrium experiments of indefinitely self-associating systems. The procedure involves application of conservation of mass criteria, along with local evaluation of the weight average molar mass, to generate a polynomial based on a composite pseudo-independent variable. The outlined method does not depend upon non-linear regression to generate a solution, but instead requires evaluation of the roots of a high-order polynomial.
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