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Updated: Feb 28, 2026
Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
MiR-495 Promotes Senescence of Mesenchymal Stem Cells by Targeting Bmi-1
Xiujun Li1, Yuxian Song2, Dan Liu1
1Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Background/Aims:
Mesenchymal stem cells (MSCs) play an important role in regulating angiogenesis and immune balance. Abnormal proliferation and function of MSCs were reported at maternal fetal interface in patients with pre-eclampsia (PE). Micro-RNA-495 was known to be upregulated in the MSCs derived from patients with PE. However, it is not clear whether the up-regulated miR-495 is related to the pathogenesis of PE.
Methods:
We analyzed the expression of miR-495 in MSCs and umbilical cords derived from healthy pregnancies (NC) and PE, then we upregulated or downregulated the expression of miR-495 in MSCs derived from NC and tested the proliferation, apoptosis, migration, invasion, tube formation and senescence.
Results:
In the current study, we found that the expression of miR-495 was significantly increased in both umbilical cord tissues and MSCs in patients with severe PE. Overexpressing miR-495 arrested cell cycle in S phase and promoted cell apoptosis. The supernatants from miR-495-overexpressed-MSCs inhibited the migration of MSCs and HTR-8/SVneo, invasion of HTR-8/SVneo and tube formation of HUVEC, while si-miR-495 had the opposite effects. Furthermore, we analyzed the senescence related β-galactosidase activity and CD146 and found that miR-495 induced the senescence of MSCs. Molecular mechanism studies confirmed that Bmi-1 mediated these effects of miR-495 on MSCs.
Conclusion:
Taken together, our data demonstrated that miR-495 induced senescence of MSCs may be involved in the pathogenesis of PE.
Insights
Elevated micro-RNA-495 (miR-495) in mesenchymal stem cells (MSCs) from pre-eclampsia (PE) patients induces MSC senescence, potentially contributing to PE pathogenesis. This study investigates miR-495
Area of Science:
- Reproductive biology
- Stem cell biology
- Molecular genetics
Background:
- Mesenchymal stem cells (MSCs) are crucial for angiogenesis and immune balance.
- Abnormal MSC function is observed in pre-eclampsia (PE).
- Micro-RNA-495 (miR-495) is upregulated in PE-derived MSCs, but its role is unclear.
Purpose of the Study:
- To investigate the role of miR-495 in the pathogenesis of pre-eclampsia (PE).
- To determine the effects of miR-495 on mesenchymal stem cell (MSC) function.
Main Methods:
- Analyzed miR-495 expression in MSCs and umbilical cords from healthy pregnancies and PE.
- Manipulated miR-495 levels in MSCs to assess proliferation, apoptosis, migration, invasion, tube formation, and senescence.
- Investigated the role of Bmi-1 in mediating miR-495 effects.
Main Results:
- miR-495 expression was significantly increased in PE patients' umbilical cords and MSCs.
- Overexpression of miR-495 induced cell cycle arrest, promoted apoptosis, and inhibited MSC and trophoblast cell migration and invasion.
- miR-495 overexpression led to MSC senescence, mediated by Bmi-1.
Conclusions:
- miR-495 induces senescence in mesenchymal stem cells.
- miR-495-induced MSC senescence is implicated in the pathogenesis of pre-eclampsia.
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