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The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection
Thomas J Gniadek1, Michael T Forman1, Isabella Martin1
1Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD.
Diagnostic Microbiology and Infectious Disease
|June 21, 2017
Abstract:
A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

