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Rainbow trout red cells in vitro.

A H Houston, C A McCullough, J Keen

    Comparative Biochemistry and Physiology. A, Comparative Physiology
    |January 1, 1985
    PubMed
    Summary
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    Rainbow trout red blood cells showed significant volume and ion changes in vitro. Pyruvate and norepinephrine partially mitigated these effects, suggesting potential stabilization strategies for fish erythrocytes.

    Area of Science:

    • * Comparative Physiology
    • * Cell Biology
    • * Aquatic Animal Health

    Background:

    • * Understanding erythrocyte function is crucial for assessing fish health.
    • * In vitro incubation can induce cellular stress and dysfunction in fish red blood cells.
    • * Ionic and osmotic balance are key indicators of erythrocyte viability.

    Purpose of the Study:

    • * To investigate the in vitro behavior of rainbow trout erythrocytes under controlled conditions.
    • * To evaluate the effects of pyruvate and norepinephrine on erythrocyte stability.
    • * To identify factors contributing to cellular dysfunction in isolated fish red blood cells.

    Main Methods:

    • * Washed rainbow trout erythrocytes were incubated at 14°C in Eagle's minimal essential medium and Cortland saline.

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  • * Cellular volume, water content, and ion concentrations (K+, Cl-, Mg2+, Na+, Ca2+) were measured.
  • * Oxygen consumption and hemoglobin system organization were assessed; pyruvate and norepinephrine were added as supplements.

    • Main Results:

    • * Incubation led to significant reductions in erythrocyte volume, water content, nucleoside triphosphate, K+, and Cl- concentrations.
    • * Mg2+ and Na+ concentrations increased, with Cl- ratios suggesting membrane hyperpolarization.
    • * Pyruvate stabilized nucleoside triphosphate, while norepinephrine stabilized Cl- content and reduced water loss and Mg2+ accumulation.

    Conclusions:

    • * Rainbow trout erythrocytes exhibit significant ionic and osmotic instability when incubated in vitro.
    • * Pyruvate and norepinephrine demonstrate potential as agents to improve the stability of fish red blood cells.
    • * Further research is needed to elucidate the mechanisms behind in vitro erythrocyte dysfunction and explore therapeutic interventions.