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Centrosomal Protein 70 Is a Mediator of Paclitaxel Sensitivity

Xingjuan Shi1, Yujue Wang2, Xiaoou Sun3

  • 1Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University, Nanjing 210096, China. xingjuanshi@seu.edu.cn.

International Journal of Molecular Sciences
|June 21, 2017
PubMed

Insights

Centrosomal protein 70 (Cep70) regulates breast cancer growth and metastasis. This study shows Cep70 mediates paclitaxel sensitivity by interacting with tubulin and promoting microtubule assembly in breast cancer.

Area of Science:

  • Oncology
  • Cell Biology
  • Pharmacology

Background:

  • Centrosome aberrations are linked to breast cancer development and progression.
  • Centrosomal protein 70 (Cep70) was previously shown to regulate breast cancer growth and metastasis.

Purpose of the Study:

  • To investigate the role of Cep70 in paclitaxel sensitivity in breast cancer.
  • To determine if Cep70 influences the efficacy of anti-microtubule chemotherapy.

Main Methods:

  • Cell proliferation assays were used to assess paclitaxel sensitivity.
  • Cep70 expression levels were altered to observe effects on drug sensitivity.
  • Mechanistic studies involving tubulin interaction and microtubule assembly assays were performed.

Main Results:

  • Cep70 expression levels positively correlated with paclitaxel sensitivity in breast cancer cell lines.
  • Altering Cep70 expression significantly modulated paclitaxel sensitivity.
  • Cep70 was found to interact with tubulin, enhancing paclitaxel-induced microtubule assembly.

Conclusions:

  • Cep70 acts as a mediator of paclitaxel sensitivity in breast cancer.
  • Cep70's interaction with tubulin and promotion of microtubule assembly are key mechanisms underlying its role in drug response.
Keywords:
Cep70breast cancermicrotubulepaclitaxel sensitivity

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