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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

6.7K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
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Centrosome Duplication02:25

Centrosome Duplication

5.1K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
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Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
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Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

282.8K
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
282.8K
Anaphase A and B01:39

Anaphase A and B

5.6K
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
5.6K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Related Experiment Video

Updated: Feb 28, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.8K

Cell Division: Centrosomes Have Separation Anxiety.

Stephen R Norris1, Ryoma Ohi1

  • 1Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.

Current Biology : CB
|June 21, 2017
PubMed
Summary

Tetrameric Kif25, a kinesin-14 motor, prevents premature centrosome separation before mitosis. This microtubule-dependent mechanism ensures proper cell division and avoids mitotic defects.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Centrosome cohesion is crucial for accurate chromosome segregation during mitosis.
  • Premature centrosome separation can lead to aneuploidy and other mitotic defects.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying centrosome tethering prior to mitosis.
  • To investigate the role of kinesin motors in maintaining centrosome integrity.

Main Methods:

  • Immunofluorescence microscopy to visualize centrosomes and microtubules.
  • Biochemical assays to study Kif25 tetramerization and microtubule binding.
  • Analysis of Kif25 knockout or knockdown cells to assess centrosome separation.

Main Results:

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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

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Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

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Last Updated: Feb 28, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

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  • Tetrameric Kif25 localizes to the centrosome and interacts with microtubules.
  • Kif25-depleted cells exhibit premature centrosome separation.
  • The microtubule-binding activity of Kif25 is essential for its function in tethering centrosomes.

Conclusions:

  • Tetrameric Kif25 acts as a key regulator of centrosome cohesion.
  • Kif25 utilizes a microtubule-dependent pathway to prevent premature centrosome separation.
  • This finding provides new insights into the maintenance of genomic stability.