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Updated: Feb 28, 2026
Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study
Published on: March 22, 2024
Evaluation of P16 expression in canine appendicular osteosarcoma
B G Murphy1, M Y Mok2, D York3
1Department Pathology, University of California, Davis, School of Veterinary Medicine, Microbiology and Immunology, Davis, CA, 95618, USA. bmurphy@ucdavis.edu.
Background:
Osteosarcoma (OSA) is a common malignant bone tumor of large breed dogs that occurs at predictable anatomic sites. At the time of initial diagnosis, most affected dogs have occult pulmonary metastases. Even with aggressive surgical treatment combined with chemotherapy, the majority of dogs diagnosed with OSA live less than 1 year from the time of diagnosis. The ability to identify canine OSA cases most responsive to treatment is needed. In humans, OSA is also an aggressive tumor that is histologically and molecularly similar to canine OSA. The expression of the tumor suppressor gene product P16 by human OSA tissue has been linked to a favorable response to chemotherapy.
Results:
We identified an antibody that binds canine P16 and developed a canine OSA tissue microarray in order to test the hypothesis that P16 expression by canine OSA tissue is predictive of clinical outcome following amputation and chemotherapy. Although statistical significance was not reached, a trend was identified between the lack of canine OSA P16 expression and a shorter disease free interval.
Conclusions:
The identification of a molecular marker for canine OSA is an important goal and the results reported here justify a larger study.
