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Updated: Feb 28, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Published on: January 3, 2015
Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9
Fatwa Adikusuma1, Nicole Williams2, Frank Grutzner3
1School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia; Centre for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang 50271, Indonesia.
Abstract:
CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.
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