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Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
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A non-invasive restraining system for awake mouse imaging.

Dan Madularu1, Axel P Mathieu2, Chathura Kumaragamage3

  • 1Department of Psychiatry, Faculty of Medicine, McGill University, Montreal, QC, Canada; Brain Imaging Centre, Douglas Mental Health University Institute, McGill University, Montreal, QC, Canada.

Journal of Neuroscience Methods
|June 22, 2017
PubMed
Summary
This summary is machine-generated.

A novel mouse restraint system enables awake preclinical neuroimaging with minimal head movement. This system enhances compatibility with various coils and allows integration with techniques like optogenetics for advanced rodent brain research.

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Area of Science:

  • Neuroimaging
  • Animal Models
  • Magnetic Resonance Imaging

Background:

  • Preclinical neuroimaging in rodents (mice, rats) assesses brain anatomy, connectivity, and function.
  • Anesthesia is commonly used to mitigate movement artifacts during scanning.
  • Anesthetized imaging presents limitations, prompting research into awake animal studies.

Purpose of the Study:

  • To introduce a novel restraining system for awake mouse neuroimaging.
  • To address limitations of existing awake imaging restraint systems.
  • To evaluate the effectiveness of the new system in reducing head motion.

Main Methods:

  • Development of a new mouse restraining system for awake imaging.
  • Evaluation of head movement in 33 awake mice during functional MRI (fMRI) scans.
  • Assessment of respiration rate and magnetic field homogeneity.

Main Results:

  • Minimal 3D linear head movement (approx. 12% of time-series) was observed in awake mice.
  • Respiration rate decreased during acclimation; bolus count remained stable.
  • Body movement did not significantly impact magnetic field (B0) homogeneity during scans.

Conclusions:

  • The novel system effectively minimizes head motion in awake mice during fMRI.
  • It offers advantages over existing systems, including coil compatibility.
  • Enables combined neuroimaging with intracranial techniques like optogenetics and microinfusion.