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Updated: Feb 28, 2026
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
Screening and identification of a specific peptide binding to cervical cancer cells from a phage-displayed peptide
Chunyan Li1, Ning Gao2, Qinqin Xue3
1Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, Shaanxi, China.
Objectives:
To screen and identify the probe markers specifically binding to human cervical cancer, a phage-displayed 12-mer peptide library was used for biopanning of SiHa cells.
Results:
After four rounds of whole-cell subtraction biopanning, the phage recovery was 21-fold higher (from 3.9 × 10-5 to 8.3 × 10-4) than that of the first round, and specific phage clones were significantly enriched. 57 randomly selected phage clones were tested by ELISA, and 36 phage clones were identified as positive clones. After sequencing of positive clones, six different peptide sequences were obtained and CSP3 showed best affinity and specificity to SiHa cells via immunofluorescence assay.
Conclusions:
Peptide, CSP3, bound to SiHa cells specifically and sensitively. It may be a potential candidate for molecular imaging detection and targeting therapy of cervical cancer.
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