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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
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The gene expression landscape of pine seedling tissues
Rafael A Cañas1, Zhen Li2,3,4, M Belén Pascual1
1Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos s/n, 29071, Málaga, Spain.
The Plant Journal : for Cell and Molecular Biology
|June 22, 2017
Summary
This study maps gene expression in maritime pine tissues, identifying core housekeeping genes and tissue-specific expression patterns. This provides a valuable resource for conifer functional genomics research.
Area of Science:
- Plant Genomics
- Molecular Biology
- Forestry Science
Background:
- Conifers are vital Northern Hemisphere resources for timber and biomaterials.
- Genomic research advances enable fundamental studies in conifers, despite experimental challenges.
- Laser capture microdissection and transcriptomic analysis are key tools for conifer tissue studies.
Purpose of the Study:
- To create a transcriptome atlas of maritime pine (Pinus pinaster) tissues.
- To identify ubiquitous and tissue-specific gene expression patterns.
- To enhance understanding of gene expression and function in conifer tissues.
Main Methods:
- Isolation and transcriptomic analysis of 14 different tissues from maritime pine seedlings.
- Utilizing laser capture microdissection for precise tissue sampling.
- Bioinformatic analysis to identify new transcripts, housekeeping genes, and expression profiles.
Main Results:
- Added 39,841 new transcripts, expanding existing sequence information.
- Identified 2,376 ubiquitous transcripts, considered core housekeeping genes.
- Clustered genes into 38 expression profiles, revealing tissue-specific expression patterns.
Conclusions:
- The study provides a comprehensive overview of gene expression distribution in pine tissues.
- Highlights the relationship between gene expression, tissue type, and function.
- The transcriptome atlas is a valuable resource for conifer functional genomics and future research.
