Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

979
The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
979
Porin Insertion in the Outer Mitochondrial Membrane01:12

Porin Insertion in the Outer Mitochondrial Membrane

4.7K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
4.7K
Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

2.3K
Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
2.3K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

16.6K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.6K
Bacterial Transformation01:33

Bacterial Transformation

59.5K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.5K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.8K
Overview
79.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A prospective, randomized clinical trial comparing plasmakinetic resection of the prostate with holmium laser enucleation of the prostate based on a 2-year followup.

The Journal of urology·2012
Same author

Wnt6 is essential for stromal cell proliferation during decidualization in mice.

Biology of reproduction·2012
Same author

Characteristics of bone tunnel changes after anterior cruciate ligament reconstruction using Ligament Advanced Reinforcement System artificial ligament.

Chinese medical journal·2012
Same author

[Expression and role of urotensin II on the lung of patients with pulmonary hypertension with congenital heart disease].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2012
Same author

A novel class of tRNA-derived small RNAs extremely enriched in mature mouse sperm.

Cell research·2012
Same author

Reciprocal regulation of Akt and Oct4 promotes the self-renewal and survival of embryonal carcinoma cells.

Molecular cell·2012

Related Experiment Video

Updated: Jan 24, 2026

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

5.1K

Engineering multi-functional bacterial outer membrane vesicles as modular nanodevices for biosensing and bioimaging.

Qi Chen1, Sharon Rozovsky, Wilfred Chen

  • 1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA. wilfred@udel.edu.

Chemical Communications (Cambridge, England)
|June 22, 2017
PubMed
Summary
This summary is machine-generated.

Engineered outer membrane vesicles (OMVs) from E. coli serve as versatile sensors. These multi-functionalized OMVs enable sensitive thrombin detection and cancer cell imaging.

More Related Videos

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
08:34

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

11.9K
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
10:21

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Published on: November 16, 2016

13.9K

Related Experiment Videos

Last Updated: Jan 24, 2026

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

5.1K
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
08:34

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

11.9K
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
10:21

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Published on: November 16, 2016

13.9K

Area of Science:

  • Biotechnology
  • Microbiology
  • Nanotechnology

Background:

  • Outer membrane vesicles (OMVs) are natural bacterial nanoparticles.
  • E. coli can naturally sort proteins into OMVs.
  • Existing biosensors have limitations in multi-functionality.

Purpose of the Study:

  • To engineer multi-functional OMV-based sensors for antigen binding and signal generation.
  • To develop a one-pot synthesis approach for OMV engineering.
  • To demonstrate OMV sensor utility in diagnostics and imaging.

Main Methods:

  • Engineered E. coli to produce OMVs with fused proteins.
  • Utilized SlyB-nanoluciferase fusion for internal signal generation.
  • Employed INP-Scaf3-Z-domain fusion for surface antibody recruitment.
  • Incorporated cohesin-dockerin system for modular functionalization.

Main Results:

  • Achieved a detection limit of 0.5 nM for thrombin detection.
  • Demonstrated successful packaging of nanoluciferase within OMVs.
  • Engineered OMVs for specific antibody binding and signal output.
  • Showcased OMV capability for cancer cell imaging using GFP.

Conclusions:

  • One-pot engineered OMVs offer a versatile platform for biosensing.
  • This approach enables sensitive detection of disease biomarkers.
  • Engineered OMVs hold potential for advanced diagnostics and targeted imaging.