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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Combining Protein and miRNA Quantification for Bladder Cancer Analysis.

Ruixue Duan¹, Zhenyu Zhang¹, Fuxin Zheng¹

¹Department of Urology, Union Hospital, Tongji Medical College, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering and Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology (HUST) , Wuhan 430074, China.

ACS Applied Materials & Interfaces

|June 22, 2017

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This study introduces a novel binary assay for simultaneously detecting telomerase and microRNA-21 (miRNA-21) in urine. The method accurately distinguishes bladder cancer patients from healthy individuals and those with cystitis.

Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Telomerase and miRNA-21 are biomarkers for various cancers, including bladder cancer.
Accurate and simultaneous detection of these biomarkers in urine is crucial for early diagnosis and treatment monitoring.
Existing methods may lack sensitivity, specificity, or the ability for simultaneous detection.

Purpose of the Study:

To develop a novel binary assay for simultaneous quantification of telomerase and miRNA-21 in urine samples.
To enhance assay sensitivity and reduce background noise for improved detection.
To evaluate the assay's performance in distinguishing between bladder cancer patients, cystitis patients, and healthy individuals.

Main Methods:

Combined telomerase extension reaction and microRNA (miRNA)-induced rolling circle amplification.

Keywords:

binary assay bladder cancer microRNA muscle-invasive bladder cancers non-muscle-invasive bladder cancers signal amplification telomerase

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

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Utilized graphene oxide (GO) and nicking enzyme-assisted signal amplification.

Employed a DNA molecular beacon probe to inhibit G-quadruplex formation and enhance telomerase activity.

Main Results:

The binary assay successfully generated distinct signals for telomerase and miRNA-21.
Graphene oxide significantly reduced background noise, improving signal-to-noise ratio.
Performance tests on 258 urine samples showed the assay could differentiate between bladder cancer, cystitis, and normal urine samples.
The assay demonstrated potential in distinguishing between muscle-invasive and non-muscle-invasive bladder cancers.

Conclusions:

The developed binary assay offers a sensitive and specific method for simultaneous detection of telomerase and miRNA-21 in urine.
This approach shows promise for early bladder cancer diagnosis and staging.
The strategy holds potential for clinical decision-making and personalized treatment strategies.