Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human milk small extracellular vesicles elicit changes in inflammatory response in infant human intestinal enteroids.

Scientific reports·2026
Same author

Intra-Abdominal Bowel Dilation in Experimental Gastroschisis is Associated with a Modifiable Transcriptomic Program of Intestinal Dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Emergence of magnetic monopole-like behavior in iron oxide nanoparticles grafted with chiral brushes: a chiral induced spin selectivity manifestation.

Materials horizons·2026
Same author

Interplay Between Exfoliation and Functionalization Strategies for Group VI Layered Transition Metal Dichalcogenide Dispersions.

Nanomaterials (Basel, Switzerland)·2026
Same author

Integrative spatial multi-omics reveal niche-specific inflammatory signaling and differentiation hierarchies in AML.

iScience·2026
Same author

CRISPR screening reveals genetic regulators associated with the evolution of eye degeneration.

bioRxiv : the preprint server for biology·2026
Same journal

Removal of Codispersible Residual Impurities from CuInS<sub>2</sub>/ZnS Quantum Dots for Window-Replaceable Luminescent Solar Concentrators.

ACS applied materials & interfaces·2026
Same journal

Durable Core-Shell Scatterer Coating with Heat Storage for Radiative Cooling.

ACS applied materials & interfaces·2026
Same journal

Calix[6]arene-Based Interlocked Inverse Vulcanizate Enabling Network-Interface Cooperative Reinforcement in Natural Rubber/Carbon Black Composites.

ACS applied materials & interfaces·2026
Same journal

Resolving Thermal Accumulation and Rigid-Soft Interface Mismatch in Stretchable Electronics with Cubic Boron Nitride Composite Islands.

ACS applied materials & interfaces·2026
Same journal

Enhancing Conversion Reversibility and Initial Coulombic Efficiency of SnO<sub>2</sub> Anodes via NiO/Ni-Carbon Interfacial Design.

ACS applied materials & interfaces·2026
Same journal

Multidimensional Interface Structure Design for High-Efficiency Optically Controlled Semiconductor Devices: A Case Study on Memristive Synapses.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

11.3K

DNA Loading and Release Using Custom-Tailored Poly(l-lysine) Surfaces.

Amin Shakiba1, Sagar L Patil2, Oussama Zenasni1

  • 1Departments of Chemistry and Chemical Engineering and the Texas Center for Superconductivity, University of Houston , Houston, Texas 77204-5003, United States.

ACS Applied Materials & Interfaces
|June 22, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed novel poly(l-lysine) (PLL) coated surfaces for efficient single-stranded DNA (ssDNA) loading and triggered release, paving the way for advanced gene delivery and biosensing applications.

Keywords:
DNAbidentateloadingpoly(l-lysine)releaseself-assembled monolayers

More Related Videos

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.5K
Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified &#955; DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

8.0K

Related Experiment Videos

Last Updated: Feb 28, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

11.3K
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.5K
Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified &#955; DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

8.0K

Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Chemistry

Background:

  • Developing functionalized surfaces is crucial for controlled biomolecule delivery.
  • Self-assembled monolayers (SAMs) offer versatile platforms for surface modification.
  • Poly(l-lysine) (PLL) is a cationic polymer suitable for DNA binding.

Purpose of the Study:

  • To create and investigate custom poly(l-lysine) (PLL) coated surfaces for single-stranded DNA (ssDNA) loading and release.
  • To explore the impact of PLL segment length on DNA loading capacity.
  • To establish a thermally triggered release mechanism for surface-bound ssDNA.

Main Methods:

  • Fabrication of azide-terminated SAMs on gold using dithiol adsorbates and oligo(ethylene glycol) (OEG) spacers.
  • Copper-catalyzed azide-alkyne cycloaddition ('click' reaction) to attach maleimide linkers.
  • Covalent attachment of cysteine-terminated PLL to maleimide-functionalized surfaces.
  • Electrostatic loading of ssDNA onto PLL-coated surfaces.
  • Thermal stimulus to induce ssDNA release.

Main Results:

  • Successfully generated stable PLL-coated surfaces via a multi-step chemical functionalization process.
  • Demonstrated efficient loading of ssDNA onto PLL SAMs through electrostatic interactions.
  • Investigated varying PLL segment lengths to optimize ssDNA loading capacity.
  • Showcased thermally triggered release of loaded ssDNA from the modified surfaces.

Conclusions:

  • The developed PLL-coated surfaces provide a promising platform for ssDNA loading and controlled release.
  • This 2D system offers preliminary insights into the potential of 3D nanostructures for gene delivery and biosensing.
  • The surface modification strategy is adaptable for future applications in nanomedicine and diagnostics.