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Related Experiment Video

Updated: Feb 28, 2026

Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton
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Hidden morphological diversity among early tetrapods.

Jason D Pardo1, Matt Szostakiwskyj2, Per E Ahlberg3

  • 1Department of Comparative Biology and Experimental Medicine, University of Calgary, 3330 Hospital Drive, Calgary, Alberta T2N 4N1, Canada.

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|June 22, 2017
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Summary

Early tetrapod evolution was more diverse than previously thought. New analysis of the aïstopod Lethiscus stocki reveals primitive features, challenging current understanding of stem tetrapod diversity and relationships.

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Area of Science:

  • Paleontology
  • Evolutionary Biology
  • Comparative Anatomy

Background:

  • Phylogenetic analyses of early tetrapod evolution suggest a homogenous stem group with gradual terrestrial adaptation.
  • The tetrapod crown group exhibits significant morphological diversity.
  • Aïstopods, like Lethiscus stocki, were previously considered highly specialized crown group members.

Purpose of the Study:

  • To re-evaluate the phylogenetic position and cranial morphology of early tetrapods, specifically the aïstopod Lethiscus stocki.
  • To challenge the existing consensus on early tetrapod evolution and stem group diversity.
  • To investigate the implications of new morphological data for phylogenetic analyses and molecular clock calibrations.

Main Methods:

  • Micro-computed tomography (micro-CT) scanning of the Lethiscus stocki specimen.
  • Digital dissection to reveal detailed cranial and braincase morphology.
  • Phylogenetic analysis incorporating new morphological data from aïstopods.

Main Results:

  • Lethiscus stocki exhibits extremely primitive cranial features, including a spiracular notch and notochord remnant.
  • Aïstopods are placed deep within the tetrapod stem group, not in the crown group.
  • Previously accepted phylogenetic relationships are challenged, with a lepospondyl lineage potentially within amniotes.
  • Stem group tetrapods show greater body plan diversity than previously assumed.

Conclusions:

  • The diversity of stem group tetrapods was underestimated.
  • Aïstopods represent a basal lineage within the tetrapod stem group.
  • Current phylogenetic frameworks and calibration dates for early tetrapod evolution require revision.
  • Character sampling is crucial for resolving early tetrapod evolutionary relationships.