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Updated: Feb 28, 2026

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

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An Experimental Framework for Quantifying Bacterial Tolerance.

Asher Brauner¹, Noam Shoresh², Ofer Fridman¹

¹The Racah Institute of Physics and the Center for NanoScience and NanoTechnology, Edmond J. Safra Campus, The Hebrew University, Jerusalem, Israel.

Biophysical Journal

|June 22, 2017

Summary

This summary is machine-generated.

This study introduces a new metric, minimum duration for killing 99% (MDK99), to measure antibiotic tolerance and persistence. This advancement aims to improve antimicrobial sensitivity testing in clinical settings.

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Area of Science:

Microbiology
Pharmacology
Clinical Science

Background:

Antibiotic tolerance and persistence are linked to treatment failure but are not well-understood or clinically measured.
Current clinical practice primarily relies on minimum inhibitory concentration (MIC) for antibiotic sensitivity, overlooking tolerance and persistence.

Purpose of the Study:

To introduce a novel metric and experimental framework for quantifying antibiotic tolerance and persistence.
To establish a standardized method for evaluating antimicrobial sensitivity beyond traditional resistance markers.

Main Methods:

Development of a new metric: minimum duration for killing 99% of the population (MDK99).
Implementation of an automated experimental framework for measuring MDK99, adaptable for manual or robotic systems.
Demonstration of the technique using various strains of Escherichia coli.

Main Results:

Successfully demonstrated a method for measuring antibiotic tolerance and persistence using the MDK99 metric.
Validated the experimental framework on Escherichia coli strains exhibiting diverse tolerance levels.
Provided a quantifiable measure for tolerance and persistence, distinct from MIC.

Conclusions:

The MDK99 metric and associated framework offer a new standard for in vitro antimicrobial sensitivity characterization.
Quantifying tolerance and persistence can provide crucial data for healthcare and advance research in antimicrobial stewardship.
Integrating MDK99 alongside MIC may lead to more effective antibiotic treatment strategies.