Primary explants as a model of the hypothalamus in situ

Anatomical and electrophysiological studies with neurotensin and somatostatin were performed to assess the integrity of primary explants of the preoptic anterior hypothalamus (POAH) maintained in organ culture as a model to study peptidergic neurons in vitro. POAH neurons maintained in vitro were found to be morphologically similar to those visualized histologically in situ. Somatostatin- and neurotensin-immunoreactive neurons were also present in both preparations. Additionally, electrophysiological responses of neurons in vitro to neurotensin and somatostatin were qualitatively similar to neuronal responses in situ. The striking morphological and pharmacological similarity between POAH neurons in vitro and in situ suggests that primary explants of the POAH are organotypic.