ANTITUMOR EFFECTS OF CHRYSANTHEMIN IN PC-3 HUMAN PROSTATE CANCER CELLS ARE MEDIATED VIA APOPTOSIS INDUCTION, CASPASE
De-Kang Sun1, Lin Wang1, Peng Zhang1
1Department of Urology Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, 264000, China.
Background:
The main objective of the current research work was to investigate the antitumor and apoptotic effects of chrysanthemin in PC-3 human prostate cancer cells.
Materials And Methods:
MTT assay was used to evaluate the effects of chrysanthemin on cell viability whereas flow cytometry along with fluorescence microscopy were used to study apoptotic induction in these cells. Effects on caspase activation were detected through western blot assay.
Results:
Results showed that chrysanthemin inhibited cancer cell growth in PC-3 cancer cells in a time-dependent as well as concentration-dependent manner. Chrysanthemin-treated cells at 10, 50 and 150 µM doses led to 34.2%, 56.7% and 69.1% apoptosis in these cells respectively. The percentage of cells with depolarized mitochondria increased from 5.3% in untreated control cells to 27.2%, 57.6% and 86.9% in cells treated with 10, 50 and 150 µM dose of chrysanthemin respectively. Chrysanthemin also enhanced the activity of all three caspases viz., caspase-3, 8 and 9 in a dose-dependent fashion.
Conclusions:
The study concluded that chrysanthemin ledanticancer effects in PC-3 prostate cancer cells by inducing apoptosis, activating caspasesignaling pathway and loss of mitochondrial membrane potential.
Insights
Chrysanthemin demonstrated significant antitumor effects against human prostate cancer cells (PC-3). This natural compound effectively induced apoptosis and activated key cell death pathways, highlighting its therapeutic potential.
Area of Science:
- * Pharmacology
- * Cancer Biology
- * Molecular Oncology
Background:
- * Prostate cancer remains a significant global health concern.
- * Investigating natural compounds for anticancer properties is crucial.
- * PC-3 cells are a widely used model for prostate cancer research.
Purpose of the Study:
- * To evaluate the antitumor activity of chrysanthemin in PC-3 cells.
- * To determine the apoptotic effects of chrysanthemin.
- * To elucidate the underlying mechanisms of chrysanthemin-induced cell death.
Main Methods:
- * MTT assay for cell viability assessment.
- * Flow cytometry and fluorescence microscopy for apoptosis analysis.
- * Western blot assay for caspase activation detection.
Main Results:
- * Chrysanthemin inhibited PC-3 cell growth in a dose- and time-dependent manner.
- * Chrysanthemin induced apoptosis in a dose-dependent manner (34.2%–69.1% at 10–150 µM).
- * Chrysanthemin treatment led to increased mitochondrial membrane potential loss and dose-dependent activation of caspases-3, 8, and 9.
Conclusions:
- * Chrysanthemin exhibits potent anticancer effects in PC-3 prostate cancer cells.
- * Apoptosis induction, caspase activation, and mitochondrial dysfunction mediate chrysanthemin's effects.
- * Chrysanthemin represents a promising candidate for prostate cancer therapy.
