Related Experiment Video
Updated: Feb 27, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Two zinc finger proteins from Mycobacterium smegmatis: DNA binding and activation of transcription
Subho Ghosh1, Dipankar Chatterji1
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.
Abstract:
Single zinc finger domain containing proteins are very few in number. Of numerous zinc finger proteins in eukaryotes, only three of them like GAGA, Superman and DNA binding by one finger (Dof) have single zinc finger domain. Although few zinc finger proteins have been described in eubacteria, no protein with single C4 zinc finger has been described in details in anyone of them. In this article, we are describing two novel C-terminal C4 zinc finger proteins-Msmeg_0118 and Msmeg_3613 from Mycobacterium smegmatis. We have named these proteins as Mszfp1 (Mycobacterial Single Zinc Finger Protein 1) and Mszfp2 (Mycobacterial Single Zinc Finger Protein 2). Both the proteins are expressed constitutively, can bind to DNA and regulate transcription. It appears that Mszfp1 and Mszfp2 may activate transcription by interacting with RNA polymerase.
Related Concept Videos
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
RNA Polymerase II Accessory Proteins
Master Transcription Regulators
Co-activators and Co-repressors
Co-activators and Co-repressors
Transcription Factors

