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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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Related Experiment Video

Updated: Feb 27, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Colloidal Gold Nanoparticles Induce Changes in Cellular and Subcellular Morphology.

Xiaowei Ma¹, Raimo Hartmann, Dorleta Jimenez de Aberasturi²

¹Chinese Academy of Sciences (CAS) Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.

|June 23, 2017

Summary

This summary is machine-generated.

Cellular morphology changes, including lysosomal swelling and cytoskeleton disruption, indicate nanoparticle toxicity. These morphological changes serve as a reliable indicator of nanoparticle harm to cells.

Keywords:

Au NPs cellular morphology cellular response cytotoxicity nanoparticles viability

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Area of Science:

Nanotoxicology
Cell Biology
Materials Science

Background:

Nanoparticle (NP) exposure can cause concentration-dependent cellular harm.
Conventional toxicity assessments often overlook NP-induced morphological changes.
Cellular morphology offers a sensitive indicator of NP effects.

Purpose of the Study:

To investigate the impact of polymer-coated gold nanoparticles (Au NPs) on cellular morphology.
To establish cellular morphology as a quantitative marker for NP toxicity.
To correlate morphological changes with NP concentration.

Main Methods:

Internalization of polymer-coated Au NPs into cells.
High-resolution imaging techniques (e.g., microscopy) for cellular analysis.
Quantitative analysis of morphological parameters (lysosomes, mitochondria, cytoskeleton, focal adhesions).

Main Results:

NP internalization led to lysosomal swelling and mitochondrial morphological alterations.
Significant disturbances in actin and tubulin cytoskeleton were observed.
Reduced focal adhesion areas and filopodia numbers were quantified.
Morphological abnormalities appeared at NP concentrations similar to or lower than those causing reduced cell viability.

Conclusions:

Cellular morphology is a sensitive and quantitative indicator of NP toxicity.
Morphological analysis provides an alternative or complementary method to biochemical assays for NP risk assessment.
Imaging and data treatment techniques enable robust quantification of NP-induced cellular changes.