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Updated: Feb 27, 2026

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass
Published on: May 27, 2022
Pharmaceutical and biomedical analysis of cannabinoids: A critical review
Cinzia Citti1, Daniela Braghiroli2, Maria Angela Vandelli2
1Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via per Monteroni, 73100 Lecce, Italy; CNR NANOTEC, Campus Ecoteckne dell'Università del Salento, Via per Monteroni, 73100 Lecce, Italy.
Abstract:
Cannabis products have recently regained much attention due to the high pharmacological potential of their cannabinoid content. In this review, the most widely used sample preparation strategies for the extraction of cannabinoids are described for the specific application to either plant materials or biological matrices. Several analytical techniques are described pointing out their respective advantages and drawbacks. In particular, chromatographic methods, such as TLC, GC and HPLC, are discussed and compared in terms of selectivity and sensitivity. Various detection methods are also presented based on the specific aim of the cannabinoids analysis. Lastly, critical considerations are mentioned with the aim to deliver useful suggestions for the selection of the optimal and most suitable method of analysis of cannabinoids in either biomedical or cannabis derived samples.
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