Related Experiment Video
Updated: Feb 27, 2026

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce Lactuca sativa Germplasm Collections
Published on: April 17, 2015
Assessment of Five Chilling Tolerance Traits and GWAS Mapping in Rice Using the USDA Mini-Core Collection
Michael R Schläppi1, Aaron K Jackson2, Georgia C Eizenga2
1Department of Biological Sciences, Marquette University, MilwaukeeWI, United States.
This study identified genetic regions (QTL) for rice chilling tolerance, crucial for developing cold-resistant crops. Findings reveal distinct genetic controls for germination and seedling stages, aiding breeding efforts.
Area of Science:
- Plant genetics
- Crop science
- Agricultural biotechnology
Background:
- Rice (Oryza sativa L.) faces chilling stress during spring planting in temperate regions.
- Improving chilling tolerance is vital for stable rice production and breeding new varieties.
- Understanding genetic pathways for chilling tolerance at germination and seedling stages is key.
Purpose of the Study:
- To dissect the genetic basis of chilling tolerance in rice at germination and seedling stages.
- To identify quantitative trait loci (QTL) associated with chilling tolerance using genome-wide association study (GWAS).
- To discover novel QTL for low temperature seedling survivability (LTSS) for marker-assisted breeding.
Main Methods:
- Developed five assays to evaluate chilling tolerance at germination and seedling stages.
- Calculated five chilling tolerance indices using 202 O. sativa accessions from the Rice Mini-Core (RMC) collection.
- Performed genome-wide association study (GWAS) for QTL mapping of chilling tolerance indices.
Main Results:
- Significant variation in chilling tolerance was observed among O. sativa accessions.
- Japonica and indica subspecies showed distinct chilling tolerance profiles at the seedling stage, with indica exhibiting acclimation potential.
- GWAS identified 48 QTL in 39 chromosomal regions, with no overlap between germination and seedling stages; two novel LTSS-QTL (qLTSS3-4, qLTSS4-1) were discovered.
Conclusions:
- Genetic architecture for rice chilling tolerance differs between germination and seedling stages.
- Identified QTL provide valuable targets for marker-assisted breeding to enhance rice chilling tolerance.
- This research contributes to gene discovery for improving rice cold hardiness and yield stability.
More Related Videos
05:22Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Published on: January 25, 2022
06:41Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Related Concept Videos
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Responses to Heat and Cold Stress
Light Acquisition