Related Experiment Videos
Thy-1: a hydrophobic transmembrane segment at the carboxyl terminus
Federation Proceedings
|October 1, 1985
Summary
Thy-1, a mammalian membrane glycoprotein, has a newly discovered 31-amino acid segment at its carboxyl terminus. This conserved segment, including a hydrophobic region, may anchor Thy-1 to the plasma membrane.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Thy-1 is a conserved membrane glycoprotein found in mammalian species.
- It is expressed in brain tissue and on cells of the hematopoietic lineage.
Purpose of the Study:
- To analyze the gene organization and protein structure of Thy-1.
- To identify novel functional domains within the Thy-1 protein.
Main Methods:
- Comparative analysis of nucleotide sequences of rat and mouse Thy-1 genes.
- Bioinformatic analysis of predicted amino acid sequences.
Main Results:
- Rat and mouse Thy-1 genes share similar organization.
- A previously undescribed 31-amino acid segment was identified at the carboxyl terminus of Thy-1.
- This segment contains a conserved 20-amino acid hydrophobic region.
Conclusions:
- The novel carboxyl-terminal segment is highly conserved across mammalian species.
- This hydrophobic segment likely plays a role in Thy-1's integration into the plasma membrane.