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Pharmacological characterization of mouse ear PCA
International Archives of Allergy and Applied Immunology
|January 1, 1985
Summary
Certain antiallergic drugs, including antihistamines and antiserotonins, effectively suppressed allergic reactions in mice. However, agents targeting slow-reacting substance of anaphylaxis (SRS-A) did not show significant effects.
Area of Science:
- Pharmacology
- Immunology
- Allergy Research
Background:
- Passive cutaneous anaphylaxis (PCA) is a model for allergic reactions.
- Understanding the mechanisms of allergic responses is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the efficacy of various antiallergic agents in a mouse ear PCA model.
- To determine which classes of antiallergic drugs are effective in suppressing allergic skin reactions.
Main Methods:
- Assessing the amount of extravasated dye in mouse ears as a measure of PCA.
- Administering different classes of antiallergic agents, including antihistamines, antiserotonins, SRS-A antagonists/inhibitors, and beta-adrenergic stimulants.
Main Results:
- Antihistamines (chlorpheniramine, diphenhydramine) and antiserotonins (methysergide, cyproheptadine) significantly suppressed PCA.
- beta-Adrenergic stimulants (isoproterenol, salbutamol) and theophylline also significantly suppressed PCA.
- SRS-A antagonist (FPL 55712) and inhibitor (AA-861), N(3',4'-dimethoxycinnamoyl)anthranilic acid (N-5'), and ketotifen showed significant suppression, while disodium cromoglycate (DSCG) did not.
Conclusions:
- Antihistamines, antiserotonins, and agents that increase cyclic AMP (cAMP) levels are effective in suppressing allergic skin reactions in mice.
- The efficacy of antiallergic agents varies, with some targeting specific pathways like SRS-A proving less effective in this model.