Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viral Mutations00:36

Viral Mutations

40.1K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
40.1K
Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

14.2K
The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
14.2K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

1.1K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.1K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

918
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
918
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

2.0K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
2.0K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

2.0K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A yellow fever 17D and Usutu virus chimera with rationally designed mutations in the envelope protein is lethal in an Ifnar<sup>-/-</sup> mouse model.

Virology journal·2026
Same author

USP24 is a cross-reactive DUB targeting MOV10 to regulate IFN-I production.

Nature communications·2026
Same author

Plug-in hybrid baculovirus expression vector for high-yield recombinant adeno-associated virus gene therapy vector production.

Trends in biotechnology·2026
Same author

Productivity and genetic stability of a novel baculovirus vector for multigene expression from independent transgene loci.

Molecular therapy. Advances·2026
Same author

SARS-CoV-2 Nsp1 suppresses the canonical NF-κB pathway by promoting ubiquitin-dependent degradation of TAK1 kinase.

PLoS pathogens·2026
Same author

4-Octyl itaconate blocks STAT3-induced ACE2 expression to reduce uptake of SARS-CoV-2.

Biochemical pharmacology·2026

Related Experiment Video

Updated: Feb 27, 2026

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.6K

Host Factors in Coronavirus Replication.

Adriaan H de Wilde1, Eric J Snijder1, Marjolein Kikkert1

  • 1Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.

Current Topics in Microbiology and Immunology
|June 24, 2017
PubMed
Summary

Coronaviruses cause severe diseases like SARS and MERS. Understanding coronavirus-host interactions is crucial for developing new treatments against these impactful pathogens.

More Related Videos

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

7.5K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.8K

Related Experiment Videos

Last Updated: Feb 27, 2026

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.6K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

7.5K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.8K

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Coronaviruses significantly impact human and animal health, causing severe respiratory and enteric diseases.
  • Notable examples include severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS).
  • Limited treatment options necessitate a deeper understanding of viral replication and host interactions.

Purpose of the Study:

  • To review current knowledge on coronavirus-host interactions within infected cells.
  • To focus on the assembly and function of the viral RNA-synthesizing machinery.
  • To examine how coronaviruses evade host innate immune responses.

Main Methods:

  • Literature review of existing research on coronavirus replication.
  • Analysis of viral proteins and their roles in host cell manipulation.
  • Examination of host-pathogen interactions at the molecular level.

Main Results:

  • Coronaviruses possess large genomes and complex expression strategies.
  • Viral proteins actively engage with host cells to facilitate replication and assembly.
  • Coronaviruses manipulate host gene expression and counteract antiviral defenses.

Conclusions:

  • Coronavirus-host interactions are central to viral pathogenesis and infection outcomes.
  • Further research into host factors is essential due to the complexity of coronaviruses.
  • Understanding these interactions is key to developing effective antiviral strategies.