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Modern Molecular Taxonomy01:29

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Using Illumina-Based Sequence Analysis to Guide Probiotic Candidate Selection and Isolation.

Wenfeng Wang1, Yi Li1, Wangsen Qin1

  • 1Department of Laboratory, Henan Provincial People's Hospital, Zhengzhou, 450003, China.

Probiotics and Antimicrobial Proteins
|June 24, 2017
PubMed
Summary

Identifying beneficial microbes from maize seeds to sprouts can improve crop health. This study found specific bacteria and fungi that enhance maize seedling growth and disease resistance.

Keywords:
AspergillusBacillusEndophyteMaizePlant probiotics

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Area of Science:

  • Microbiology
  • Plant Science
  • Agricultural Science

Background:

  • Selecting effective probiotic candidates for agricultural applications is challenging and resource-intensive.
  • Understanding the microbial communities associated with crop plants, like maize, is crucial for developing sustainable agricultural practices.

Purpose of the Study:

  • To identify microbial taxa (bacteria and fungi) transmitted from maize seeds to seedlings during germination.
  • To evaluate the probiotic effects of these identified microbes on maize growth and disease resistance.

Main Methods:

  • Illumina-based sequencing was employed to analyze bacterial and fungal taxa in maize kernel germs and sprouts.
  • Isolation and testing of specific spore-forming bacteria (BS3) for their impact on maize seedling growth and resistance to Fusarium verticillioides.

Main Results:

  • Fungal diversity increased, while bacterial species diversity decreased during maize germination from seed to sprout.
  • Dominant seed-borne genera (Bacillus, Halomonas, Shewanella, Aspergillus) were found in sprouts.
  • Isolated spore-forming bacteria BS3 promoted maize seedling growth and enhanced resistance to F. verticillioides.

Conclusions:

  • Maize harbors core bacterial and fungal taxa during its development from seed to sprout.
  • These core endophytic microbes demonstrate a strong association with the host plant.
  • Illumina sequencing is a viable method for guiding the selection and isolation of microbial probiotic candidates.