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Updated: Feb 27, 2026
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Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
Published on: June 14, 2012
26.2K
Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai1,2, Takashi Yamamoto3
1PRESTO, JST, Higashi-Hiroshima, 739-8530, Japan. ochiai@hiroshima-u.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2017
Abstract:
Zinc-finger nucleases (ZFNs) are programmable nucleases that have opened the door to the genome editing era. The construction of ZFNs recognizing a target sequence of interest is laborious, and has not been widely used recently. However, key ZFN patents are expiring over the next 2-4 years, enabling a wide range of deployments for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-stranded annealing (SSA) assay.
