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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Oct 2, 2025

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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CRISPR/Cas9.

Izuho Hatada1, Takuro Horii2

  • 1Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Gunma, Japan. hatada@gunma-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2017
PubMed
Summary

CRISPR/Cas9 gene editing is a widely adopted technology due to its simplicity. This study details vector construction and target gene editing using the CRISPR/Cas9 system in cells.

Keywords:
CRISPR/Cas9Cas9gRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a revolutionary genome editing tool.
  • Its simplicity and ease of use have led to global adoption.
  • Efficient genome editing is crucial for biological research and therapeutic development.

Purpose of the Study:

  • To describe the construction of vectors for CRISPR/Cas9 system.
  • To demonstrate genome editing of a target gene in cells.
  • To provide a practical guide for implementing CRISPR/Cas9 technology.

Main Methods:

  • Vector construction for CRISPR/Cas9 components.
  • Introduction of CRISPR/Cas9 system into target cells.
  • Validation of genome editing at the target locus.

Main Results:

  • Successfully constructed functional CRISPR/Cas9 vectors.
  • Demonstrated efficient editing of the target gene in cellular models.
  • Confirmed successful integration and expression of the CRISPR/Cas9 system.

Conclusions:

  • The described methods facilitate straightforward CRISPR/Cas9 genome editing.
  • This approach is effective for modifying target genes in cells.
  • The study provides a foundation for further applications of CRISPR/Cas9 technology.