Related Experiment Video
Updated: Feb 27, 2026
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation
Published on: March 15, 2024
Genome Editing of Rat.
1Division of Science and Engineering, Graduate School of Arts and Science, Iwate University, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan. takekaneko5@gmail.com.
Genome editing techniques like CRISPR-Cas9 enable rapid production of genetically engineered rats for research. New methods, including the Technique for Animal Knockout system by Electroporation (TAKE), simplify creating these valuable animal models.
Area of Science:
- Biomedical research
- Genetics
- Animal models
Background:
- Genetically engineered rat strains are crucial for biomedical research.
- Genome editing technologies have revolutionized the creation of custom animal models.
Purpose of the Study:
- To introduce the latest protocols for producing genetically engineered rats.
- To highlight efficient genome editing techniques for rat model generation.
Main Methods:
- Utilizing genome editing tools such as zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR-Cas9.
- Employing embryo microinjection and a novel electroporation method, Technique for Animal Knockout system by Electroporation (TAKE).
Main Results:
- Demonstration of simple and quick production of knock-out and knock-in rats.
- Successful generation of genome-edited animals using advanced techniques.
Conclusions:
- Genome editing offers efficient strategies for creating genetically engineered rats.
- The described protocols facilitate the development of novel rat models for diverse research applications.
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