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In-vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Feb 27, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

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Genome Editing of Pig.

Masahito Watanabe1,2, Hiroshi Nagashima3,4

  • 1Meiji University International Institute for Bio-Resource Research, Kawasaki, 214-8571, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2017
PubMed
Summary

Researchers developed a streamlined workflow for creating gene knock-out pigs, utilizing advanced genome editing tools and somatic cell nuclear transfer. This method efficiently generates genetically modified pigs for biomedical research and translational studies.

Keywords:
CloningCytoplasmic injectionGene knockoutPigSomatic cell nuclear transfer

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Area of Science:

  • Animal biotechnology
  • Genomic research
  • Translational medicine

Background:

  • Pigs serve as valuable biomedical models due to their physiological and anatomical resemblance to humans.
  • Advancements in genome editing technologies (ZFN, TALEN, CRISPR/Cas9) have enabled precise genetic modifications in various species.
  • There is a growing demand for genetically modified pigs in research settings.

Purpose of the Study:

  • To introduce an efficient workflow for generating gene knock-out pigs.
  • To detail the process of establishing genetically modified nuclear donor cells.
  • To focus on the practical generation of gene knock-out pigs via cytoplasmic injection.

Main Methods:

  • Utilized somatic cell nuclear transfer (SCNT) for generating gene knock-out pigs.
  • Established genetically modified cell lines (nuclear donor cells) using advanced genome editing techniques.
  • Employed a cytoplasmic injection method for the efficient generation of modified embryos.

Main Results:

  • Successfully established a workflow for creating genetically modified nuclear donor cells.
  • Demonstrated the efficient generation of gene knock-out pigs using the described SCNT and cytoplasmic injection methods.
  • Contributed to the increasing number of reports on genetically modified pig development.

Conclusions:

  • The presented workflow facilitates the efficient generation of gene knock-out pigs.
  • This methodology supports the advancement of pigs as experimental models in biomedical research.
  • The study highlights the impact of genome editing on livestock genetic modification.